TABLE 1.

Summary of the properties of WT (Cx32*43E1) and mutant hemichannels

Channel	Vj-gating polarity	Slope conductance in 100 mM KCl	Direction of open-channel current rectification
WT	Negative	115 pS	Inward
N2E	Positive	260 pS	Slightly outward
G5D	Positive	240 pS	Linear
T8D	Bipolar*	180 pS	Linear
N2R/K	Negative	70 pS	Inward
G5R/K	Negative	70 pS	Inward
T8R/K	Negative	80 pS	Inward
N2E+G5K	Bipolar*	120 pS	Inward
N2R+G5D	Bipolar†	105 pS	Linear
N2E+G5R	Bipolar (?)‡	120 pS	Inward

^*V_j-gating more sensitive to positive potentials.

^†V_j-gating more sensitive to negative potentials.

^‡The high incidence of loop gating at negative potentials precludes the assignment of bipolarity to this channel. V_j-gating events are more prevalent at positive potentials.