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. 2004 Sep;87(3):1672–1685. doi: 10.1529/biophysj.104.043174

FIGURE 3.

FIGURE 3

Effects of PEGs added only from the intracellular side on single maxi-anion channel currents. (A) Representative single-channel events recorded in the absence (left panel) and in the presence of PEG 200 (middle panel) or PEG 4000 (right panel) in the bath. Pipettes were filled with control Ringer solution, and the maxi-anion channels were activated by excising the patch into the control Ringer solution before challenging it with the test polymer-containing solution. (B) Current-voltage relationships for the maxi-anion channel in control symmetrical control Ringer solution (left panel) and with PEG 200 (middle panel) or PEG 4000 (right panel) present in the bath. Each symbol represents the mean value of 5–20 observations obtained from at least five different patches. Solid lines are linear fits with slopes corresponding to the unitary inward conductances of 402.8 ± 8 pS, 297 ± 13 pS, 395.3 ± 6.1 pS, and outward conductances of 400 ± 8.0 pS, 366.8 ± 5.0 pS, and 409.0 ± 6.0 pS for control, PEG 200 and PEG 4000, respectively.