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Peptides observed by LC/MS of pepsin digests of soluble state SC3 (A), soluble state PFA-SC3 (B), PFA-SC3 in α-helical state (C), and PFA-SC3 in β-sheet state (D). Arrows in the SC3 sequence indicate the assigned peptides. The dotted rectangles highlight the predicted second loop of SC3. The asterisks indicate the oxidized cysteines upon PFA treatment.
