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MALDI-TOF determination of the peptides generated by endoproteinase Asp-N
| Peptide | Theoretical mass (Da) | Measured m/z | Number of clustered sodium | Theoretical m/z | |
|---|---|---|---|---|---|
| 1 | C*39-S63 | 2555.3 | 2622.7 | 3 | 2622.3 |
| 2 | D64-S72 | 928.5 | 951.4 | 1 | 951.5 |
| 3 | C*73-G85 | 1193.6 | 1238.4 | 2 | 1238.6 |
| 4 | C*93-G105 | 1469.7 | 1514.4 | 2 | 1514.7 |
| 5 | C*106-L112 | 820.4 | 865.3 | 2 | 865.4 |
The asterisks indicate the oxidized cysteines upon PFA treatment.
All the peptide masses listed are monoisotopic masses.
