TABLE 2.

Distribution of rate constants (k, min⁻¹) for H/D exchange at amide bonds in peptic peptides generated from soluble-state SC3

		Number of amide hydrogens*
Peptide	Sequence	NHs	Fast (k > 1000)	Slow (100 > k > 1)	Very slow (0.1 > k)
S47-L54	SSSPVTAL	6	3.6	2.0	0.40
L54-L57	LLGL	3	1.1	1.2	0.7
L55-G59	LGLLG	4	1.6	1.7	0.7
L58-L62	LGIVL	4	2.6	1.3	0.1
I60-L62	IVL	2	1.1	0.9	0
I60-L65	IVLSDL	5	4.6	0.3	0.1
S63-V67	SDLNV	4	2.8	0.7	0.5
N66-G70	NVLVG	4	1.1	2.1	0.8
N66-L68	NVL	2	0	1.8	0.2
L76-S84	LTVIGVGGS	8	7.4	0	0.6

^*The number of peptide amide hydrogens that undergo fast, slow, very slow deuterium exchange were obtained with Eq. 2 and are described by N1, N2, and N3, respectively. NHs indicates the number of possible exchanged amide hydrogens; k indicates the rate constant (min⁻¹) obtained from Eq. 2.