TABLE 3.
Distribution of rate constant (k, min−1) for H/D exchange at amide bonds in peptic peptides generated from Teflon-associated α-helical-state PFA-SC3
| Number of amide hydrogens*
|
|||||
|---|---|---|---|---|---|
| Peptide | Sequence | NHs | Fast (k > 1000) | Slow (100 > k > 1) | Very slow (0.1 > k) |
| C*39-Q44 | C*C*NQVQ | 5 | 1.5 | 2.1 | 1.4 |
| A46-L54 | ASSSPVTAL | 7 | 0.6 | 0.7 | 5.7 |
| L54-L57 | LLGL | 3 | 0 | 0.2 | 2.8 |
| L55-G59 | LGLLG | 4 | 1.3 | 0.3 | 2.4 |
| L58-L62 | LGIVL | 4 | 0.5 | 3.0 | 0.5 |
| V61-N66/L62-V67 | VLSDLN/LSDLNV | 5 | 0 | 3.5 | 1.5 |
| L65-S74 | LNVLVGISC*S | 9 | 3.7 | 0.3 | 5.0 |
| N66-G70 | NVLVG | 4 | 0.3 | 1.3 | 2.4 |
| N66-L68 | NVL | 2 | 0.4 | 1.0 | 0.6 |
| 171-179 | ISC*SPLTVI | 7 | 4.2 | 0.8 | 2.0 |
| 179-Q89 | IGVGGSGC*SAQ | 10 | 0.7 | 1.1 | 8.2 |
| L101-L112 | LINIGC*TPINIL | 10 | 0 | 3.6 | 6.4 |
The asterisks indicate the oxidized cysteines upon PFA treatment.
*
See Table 2.