TABLE 4.
Distribution of rate constant (k, min−1) for H/D exchange at amide bonds in peptic peptides generated from Teflon-associated β-sheet-state PFA-SC3
| Number of amide hydrogens*
|
|||||
|---|---|---|---|---|---|
| Peptide | Sequence | NHs | Fast (k > 1000) | Slow (100 > k > 1) | Very slow (0.1 > k) |
| C*39-Q44 | C*C*NQVQ | 5 | 0.7 | 1.4 | 2.9 |
| A46-L54 | ASSSPVTAL | 7 | 0.1 | 0.7 | 6.2 |
| P50-L54 | PVTAL | 3 | 0.3 | 0.5 | 2.2 |
| L54-L57 | LLGL | 3 | 0 | 0 | 3.0 |
| L55-G59 | LGLLG | 4 | 0 | 0 | 4.0 |
| L58-L62 | LGIVL | 4 | 1.2 | 06 | 2.2 |
| I60-L62 | IVL | 2 | 0 | 0.3 | 1.7 |
| V61-N66/L62-V67 | VLSDLN/LSDLNV | 5 | 0 | 4.6 | 0.4 |
| L65-S74 | LNVLVGISC*S | 9 | 1.4 | 3.4 | 4.2 |
| N66-L68 | NVL | 2 | 0 | 1.1 | 0.9 |
| V69-T77 | VGISC*SPLT | 7 | 0 | 2.4 | 4.6 |
| 171-V78 | ISC*SPLTV | 6 | 0 | 4.2 | 1.8 |
| L101-L112 | LINIGC*TPINIL | 10 | 5.5 | 4.4 | 0.1 |
| I102-L112 | INIGC*TPINIL | 9 | 4.7 | 4.0 | 0.3 |
The asterisks indicate the oxidized cysteines upon PFA treatment.
*
See Table 2.