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DNA (1000 μM) and ethidium bromide (50 μM). (a) Absorption spectra of ethidium bromide (dashed line) and ethidium bromide-ct-DNA (solid line). (Inset) Enlarged region of ethidium bromide band. (b) Fluorescence excitation spectra with all emitted photons detected, (c) LD spectra of ct-DNA (dashed line) and ethidium bromide-ct-DNA (solid line), and (d) FDFLD spectra. All solutions prepared using a sodium cacodylate buffer (10 mM, pH 7).
