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. 2004 Sep;87(3):2043–2059. doi: 10.1529/biophysj.103.038497

FIGURE 2.

FIGURE 2

The number of complexes formed in BD simulations of Phormidium cyt f and Phormidium PC under different conditions. (A) The effect of mutation of anionic residues on Phormidium PC on its interaction with Phormidium cyt f. (○) WT PC minus Zn2+; (×) D45A-PC minus Zn2+; (+) D44A + D45A-PC minus Zn2; (□) WT-PC plus Zn2+; (•) D44A + D45A + E54A-PC minus Zn2+.; (*) D44A + D45A + D57A-PC minus Zn2+; and (▪) D44A + D45A + E54A + D57A-PC minus Zn2+. (B) Comparison of cyt f-PC interactions of cyanobacteria with those of green algae. Conditions were as described in the Methods section. (•) Chlamydomonas cyt f interacting with Chlamydomonas PC; (▪) Phormidium cyt f interacting with Phormidium PC in the presence of Zn2+; (*) Phormidium cyt f interacting with Phormidium PC in the presence of Zn2 + in the absence of an electrostatic field; (□) Phormidium cyt f interacting with Chlamydomonas PC; and (○) Chlamydomonas cyt f interacting with Phormidium PC + Zn2+. (C) The effect of mutation of cationic residues on Phormidium PC in the presence of Zn2+ on the number of complexes formed with Phormidium cyt f. (▪) WT-PC; (○) K6A-PC; (•) K35A- PC; (*) K46A-PC; (□) R93A-PC; and (X) R93E-PC. (D) The effect of mutations of Phormidium cyt f on its ability to interact with Phormidium PC in the presence of Zn2+. (▪) WT-cyt f; (□) D42A-cyt f; (•) D63A-cyt f; (*) D187A-cyt f; (○) D122A-cyt f; and (X) E95A-cyt f.