FIGURE A3.

Rate of generation as function of NADH concentration. (a) The rate of production at Complex I was modeled according to Kushnareva et al. (2002). In the calculation, we assume that most of the electron donor is in the nonreduced state and that there is an equilibrium relationship between the NADH/NAD⁺ couple and the electron donor site in its reduced and oxidized state (see Eqs. A1 and A2). The equilibrium constant between NADH and the electron donor was considered equal to 0.01 and the rate constant k′ = 1.0 mM⁻¹ s⁻¹. (b) The rate of production was studied as a function of ΔΨ_m according to the minimal model described in Demin and co-workers (reproduced from Demin et al., 1998, Mol. Cell Biochem., with permission).