TABLE 1.

Main transition temperature (T₀) and enthalpy (ΔH, ΔH_vH)

Additives to imaging buffer	T0 (K)	ΔHvH (kJ mol−1)	ΔH (kJ mol−1)
0 mM heptanol	296.5	−2928	−15.0
1 mM heptanol	295.8	−4487	−23.0
2 mM heptanol	294.1	−6087	−31.2
3 mM heptanol	291.3	−8570	−44.0
0 mM hept., washout	296.7	−2439	−12.5
5 mM CaCl2	297.5	−2004	−10.3

T₀ and ΔH_vH were derived from a fit of the data given in Fig. 6 by Eq. 4. Using a cooperative unit n_c = 195 molecules (see text) ΔH of single molecules was calculated by Eq. 5.