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(A) Initial polymerization of RecA on a DNA molecule unwound by 580 turns (
), in the presence of ATPγS. (B) Initial polymerization of RecA on a DNA molecule unwound by 400 turns (
), in the presence of ATP (arbitrary time origin). The external force applied is ∼6 pN for both molecules. Fluctuations are observed to be considerably larger in the ATP case, which hints at a very rich dynamics via ATP hydrolysis.
