TABLE 2.

Fluorescence lifetime constants (τ_i) and fractional contributions (α_i) of wild-type E. coli GR

Potassium phosphate				MOPS
wt GR		wt GR + NADP+		wt GR		wt GR + ADPR
τi (ns)	αi	τi (ns)	αi	τi (ns)	αi	τi (ns)	αi
0.007 ± 0.001	0.90	0.007 ± 0.002	0.76	0.007 ± 0.001	0.82	0.005 ± 0.001	0.67
0.090 ± 0.008	0.076	0.12 ± 0.01	0.18	0.061 ± 0.009	0.13	0.11 ± 0.03	0.15
0.29 ± 0.04	0.020	0.37 ± 0.04	0.04	0.22 ± 0.02	0.04	0.34 ± 0.02	0.10
1.0 ± 0.2	0.003	0.76 ± 0.05	0.01	0.86 ± 0.2	0.004	0.73 ± 0.1	0.053
2.6 ± 0.3	0.002	2.1 ± 0.2	0.01	2.4 ± 0.1	0.002	2.0 ± 0.4	0.013

At 293 K, pH 7.6, in 50 mM potassium phosphate buffer, unliganded and saturated with NADP⁺; and 50 mM MOPS buffer, unliganded and saturated with 2′P-5′ADP-ribose (ADPR).