FIGURE 5.

Representative time-resolved data accounting for a single APC trimer in PVA. (A) Isotropic fluorescence intensity and (B) fluorescence lifetime trajectories. Lifetimes were estimated by MLE fitting each 1000 photons over the single-molecule fluorescence trajectory. (C) Total fluorescence decay constructed from the single-molecule fluorescence trajectory. (D) Unquenched and (E) quenched single-molecule fluorescence decays (1000 photons each) observed during the measurement of the single APC trimer. (F) Histogram of lifetimes from panel B and Gauss fits. (G) Intensity versus lifetime correlation constructed from panels A and B.