TABLE 1.
Relationship between plating and surface density for both fibronectin (FN) and GRGDSP (RGD)
| Concentration
|
Number of cells
|
|||
|---|---|---|---|---|
| Substrate | Plating | σ | 〈A〉, 〈|F|〉, 〈|T|〉 | 〈S〉 |
| (μg/ml) | (Molecules/μm2) | n | n | |
| FN | 0.5 | 10 | - | 9 |
| FN | 1.56 | 30 | - | 16 |
| FN | 3.13 | 60 | 8 | - |
| FN | 6.25 | 120 | 14 | - |
| FN | 12.5 | 240 | 15 | 27 |
| FN | 25 | 475 | 24 | 35 |
| FN | 50 | 950 | 21 | 37 |
| FN | 100 | 1900 | 15 | 40 |
| RGD | 25 | 50 | 13 | 23 |
| RGD | 50 | 100 | 12 | 25 |
| RGD | 100 | 200 | 10 | 25 |
| RGD | 250 | 500 | 17 | 27 |
| RGD | 500 | 1000 | 8 | 30 |
| RGD | 750 | 1500 | 6 | - |
Polyacrylamide substrata were modified with indicated plating concentrations of FN or RGD. The immobilized surface density (σ) of FN or RGD on the substratum is given for each plating concentration. The total number of cells for each measurement is indicated by n. 〈|T|〉, 〈|F|〉, 〈A〉, and 〈S〉 are the population-averages of the traction magnitude, total absolute force, projected area, and migration speed, respectively.