TABLE 1.
Values of equilibrium constants used in energy inventory
| Symbol | Reaction | Value | Reference |
|---|---|---|---|
| Actin | |||
| Kx | Nucleotide exchange | 6* | Kinosian et al. (1993) |
| Kp | Pi binding to filaments | 1.5 μM | Carlier and Pantaloni (1988) |
| [MT](+)-crit | MT addition to (+)-ends | 0.1 μM | Pollard et al. (2000) |
| [MD](−)-crit | MT addition to (−)-ends | 1.7 μM | Pollard et al. (2000) |
| Tubulin | |||
| Kx | Nucleotide exchange | 3* | Zeeberg and Caplow (1979) |
| Kp | Pi binding to filaments | 25 mM | Carlier et al. (1988) |
| [MT](+)-crit | MT addition to (+)-ends | 0.03 μM | Howard (2001) |
| [MD](−)-crit | MT addition to (−)-ends | 100 μM† | Howard (2001) |
Calculated from the ratio of measured equilibrium dissociation constants of nucleotide binding to the monomer, i.e., Kx = KNDP/KNTP.
Assumed equal to (+)-end critical concentration for tubulin·GDP.