TABLE 1.

Summary of effects of Mg²⁺, Mn²⁺, and Ni²⁺ on wild-type and mutant eag channels

	Half-maximal concentration (mM)			Peak fold change in t½
	Mg2+	Mn2+	Ni2+	Mg2+	Mn2+	Ni2+
Wild-type	3.5	6.2	0.12	6.7	6.6	123
D274A	5.4	6.3	0.0022*, 0.0015**	9.3	9.1	NA†
D274E	1.2	2.6	ND‡	22.8	18.5	ND
D278V	—§	—	—	—	—	—
D278E	—	17.7	—	—	5.3	—
D327A	—	—	0.37	—	—	93

^* Determined from current decline.

^** Estimated K_D from on/off rates.

^† Not applicable.

^‡ Not determined.

^§ Insensitive.