FIGURE 8.

(A) Trimeric conductance (g) of WT and (L)EAE in mixtures of NaCl and CaCl₂, pH 7.4 (with Na⁺ + Ca²⁺ kept constant at 0.1 M), as a function of the mole fraction of Ca²⁺ (symbols). Each data point is based on at least five independent measurements. Curves represent simulated DFT-PNP data. The data points plotted over the left end of the abscissa were obtained with solutions that contained no added Ca²⁺. The Cl⁻ content of the solutions experimentally tested increased from left to right from (in mM) 74.1 (Ca²⁺ MF = 0) to 145.3 (Ca²⁺ MF = 1). (B) Number of Ca²⁺ and Na⁺ ions that on average occupy the pore of a single monomer in relation to the Ca²⁺ mole fraction.