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TEA interferes with MTSET modification of C425 in Shaker. (A) MTSET (300 μM) was applied in the absence (shaded circles) and presence (black circles) of 100 mM TEA. Data points represent mean ± SE of 3–4 cells. (B) Composite data for three conditions, including the wild-type channel (WT), which lacked the cysteine in the outer vestibule. All cells were preincubated for 3 min with 10 mM DTT.
