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Membrane binding of 3H-NEM-SCAMP E-peptide (+4) to PC vesicles (○), 1% PI(4,5)P2 in PC (▴), and 3% PI(4,5)P2 in PC (•) as a function of the concentration of accessible lipid. The reciprocal molar binding constants for PC, 1% PI(4,5)P2, 3% PI(4,5)P2 are 130, 8.9 × 102, and 1.4 × 104 M−1, respectively. The radiolabeled peptide is at a concentration of 40 nM. The membrane vesicles are extruded into a buffer of 100 mM KCl, 1 mM MOPS, pH 7.0.
