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Membrane depths for spin labels on SCAMP-E peptide
| PC/PS (3:1)
|
PC/PS/PI(4,5)P2 (73:22:5)
|
|||
|---|---|---|---|---|
| Peptide | Φ | d (Å) | Φ | d (Å) |
| C1R1 | 0.64 | 7.3 | 0.82 | 7.8 |
| I6R1 | 0.76 | 7.6 | 1.3 | 9.1 |
| A9R1 | 1.2 | 8.8 | 1.2 | 8.8 |
The membrane depth, d, corresponds to the position of the nitroxide side chain from the level of the lipid phosphate where positive values indicate a position within the bilayer. The error in the depth due to the error in Φ is ∼±1 Å.
