FIGURE 3.

Data (points) and fits (lines) for equilibrium AUC measurements at 40, 45, and 50 KRPM for the following MS1-Gly⁴ peptides in 4 mM C14-betaine at 1:200 total peptide/detergent ratio: (A) NBD-labeled (ɛ₄₇₅ = 84); (B) TMR-labeled (ɛ₃₆₀ = 30); and (C) 1:1 molar mixture of NBD-and TMR-labeled (ɛ₄₇₅ = 50 based on measured spectrum of mixture). Extinction coefficient units are (mole ratio)⁻¹ cm⁻¹. Fits were done using a monomer-dimer equilibrium model.