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NSOM fluorescence images of 40-nm fluorescent spheres embedded in a 40-nm layer of PVA for zero separation (left column) and different interleave heights (right column): 30, 50, 100, 200, and 300 nm. The excitation wavelength is 568.5 nm, objective 100× oil immersion, 1.3 NA, and probe aperture size ∼60 nm.
