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. 2004 Aug 17;87(5):3536–3546. doi: 10.1529/biophysj.104.044404

FIGURE 2.

FIGURE 2

Calibration of the system against a laser Doppler velocimeter (LDV) in the plane of microscope focus. The target was a piece of scratched Plexiglas glued to the end of a piezoelectric crystal. The microscope was focused on the center of the Plexiglas at the same time that the LDV was aimed at a piece of retroreflective tape on the end of the Plexiglas. Circles show five independent measurements of motion at each of 20 stimulus intensities. The straight line has a slope of one. The maximum error in the CMV measurement is a consistent 5–10% underestimate at larger displacements introduced by the motion measurement algorithm.