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1P (A) and 2P (B) spectra of myosin labeled at RLC with rhodamine. 1P and 2P of Rh-RLC-myosin and Rh-RLC-myosin in the presence of equimolar actin were the same. Final concentration of Rh was 0.25 μM, temperature 20°C. M, myosin; A, actin; glyc, glycerol.
