TABLE 1.
Lifetimes of Rh, Rh-RLC, and Rh-RLC on myosin and Rh-RLC on myosin + actin measured using fluorescence lifetime spectrometer FluoTime 100
| Compound |
 (ns)*
|
αi | τi (ns) |  |
|---|---|---|---|---|
| Rh-RLC | 4.63 | 0.586 | 2.05 | 1.22 |
| 0.414 | 5.90 | |||
| Rh-RLC on myosin | 3.60 | 0.542 | 1.53 | 1.37 |
| 0.458 | 4.44 | |||
| Rh-RLC on myosin + actin | 3.58 | 0.563 | 1.75 | 1.38 |
| 0.437 | 4.50 | |||
| Rh | 2.33 | 0.429 | 1.25 | 1.41 |
| 0.571 | 2.70 |
Excitation 442 nm was from diode laser system (PTD 800B with LDH PC 440). Data analysis was performed using multiexponential fluorescence decay fitting software FluoroFit version 3.2.0.
*
