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Apparent pKa values for site N3 of the protonatable cytosine bases for the hairpin during folding (at pH 4.5)
| Site |
 (10a) |
 (10a) |
 (10b) |
 (10c) |
 (10d) |
 |
 |
|---|---|---|---|---|---|---|---|
| C18 | 5.2 | 5.1 | 5.2 | 5.3 | 6.3 | 6.5 | 6.4 |
| C21 | 5.4 | 4.7 | 5.1 | 5.4 | 5.8 | 5.9 | 5.9 |
| C22 | 5.5 | 4.9 | 5.6 | 6.0 | 6.3 | 6.4 | 6.4 |
| C7 | 4.2 | 4.2 | 4.2 | 4.3 | 4.3 | 4.4 | 4.4 |
The pKapp values for the protonated sites are shown in bold (the notation corresponds to that in Eq. 15).
