TABLE 1.

Statistics for crystal structures of staphylococcal nuclease variants

Crystal structure label	C1	C2	C3	C4*	C5	C6
SNase variant	Wild-type	PHS	Δ+PHS/V66K	WT/V66K	PHS/V66E	PHS/V66E
PDB accession code	1EY0	1EY8	—	2SNM	—	1U9R
Temperature (K)	297	297	95	298	95	296
pH	7.0	7.0	8.0	8.0	6.0	6.4
Resolution limit (Å)	1.60	1.75	2.1	1.97	2.1	2.1
R-value	0.20	0.19	0.19	0.18	0.19	0.19
Free R-value	0.26	0.24	0.22	—	0.27	0.22
Residues	6–141	7–141	7–43, 50–141	7–141	7–141	7–44, 51–141
Non-hydrogen protein atoms	1091	1076	1025	1085	1079	1031
Water oxygen atoms	82	74	19	41	64	31
Median B-factor for protein atoms (Å2)	34.9	34.4	31.1	22.9	32.6	32.1
Median B-factor for water oxygens (Å2)	48.8	50.7	27.2	34.1	30.8	37.3
Reference	Chen et al., 2000	Chen et al., 2000	García-Moreno et al., 1997	Stites et al., 1991	Dwyer et al., 2000	This work

^*This structure also contains a Ca²⁺ ion and a 2′-deoxythymidine 3′,5′-diphosphate inhibitor in the active site.