TABLE 1.

The B₂₂ values of various proteins under different solution conditions, as determined by the method presented in this study compared to those reported in literature

	B22 (10−4 mol ml/g2)
Solution conditions (pH, NaCl concentration)	Our results (mean ± SD, n = 3)	Literature values
Lysozyme
pH 4.6, 40 mM	2.6 ± 0.1	3.8*
pH 4.6, 150 mM	1.0 ± 0.2	2.0
pH 4.6, 400 mM	−1.8 ± 0.1	−0.8
pH 4.6, 1.14 M	−3.0 ± 0.3	−2.1
Chymotrypsinogen
pH 3.0, 40 mM	0.9 ± 0.1	6.0†
		1.9‡
pH 3.0, 100 mM	0.1 ± 0.1	1.5
		1.8
pH 3.0, 200 mM	−1.6 ± 0.2	—
pH 3.0, 300 mM	−1.6 ± 0.2	−1.5
		0.5
pH 5.0, 300 mM	−1.6 ± 0.2	−1.2
pH 6.8, 300 mM	−1.2 ± 0.2	−2.0
Chymotrypsin
pH 3.0, 40 mM	0.8 ± 0.1	—
pH 3.0, 100 mM	0.1 ± 0.01	—
pH 3.0, 200 mM	−3.3 ± 0.3	—
pH 3.0, 300 mM	−5.8 ± 0.3	—

^*Rosenbaum and Zukoski (1996).

^†Velev et al. (1998).

^‡Tessier et al. (2002).