TABLE 2.

Average effective diameter of Hemoglobin A and S in different phosphate buffers from DLS measured at 90°

Hb in different buffers	D (nm)
HbA in PBS	6.5 ± 0.1
HbA in 1.5 M phosphate	300 ± 66
HbA in 1.8 M phosphate	402 ± 33
HbA in 1.8 M phosphate at 37°C	650 ± 73
HbS in PBS	6.5 ± 0.1
HbS in 1.5 M phosphate	540 ± 56
HbS in 1.8 M phosphate	822 ± 116
HbS in 1.8 M phosphate at 37°C	1368 ± 305
Deoxy HbS solution in 1.8 M phosphate at 15°C	1767 ± 170