Figure 2.

Conditional deletion of Eif4g2 specifically impairs SP thymocyte development

(A and B) Validation of eIF4G2 protein deletion by western blot. (A) Total thymocytes.

(B) Lysates from sorted thymocyte subsets: double-negative (DN), double-positive (DP), and single-positive (SP) cells.

(C) Representative images of thymus from WT and Eif4g2 cKO mice.

(D) Total thymocytes numbers from WT and Eif4g2 cKO mice (n = 3 per group, ns p > 0.05).

(E) Flow cytometric analysis of thymocyte populations.

(F–H) Relative frequencies and absolute numbers of (F) CD4 SP, (G) CD8 SP, and (H) TCRβ⁺CD8⁺ subsets (n = 3–6 mice per group, presented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.001).

(I–M) Frequencies of (I) DN, (J) DP cells within total thymocytes, and (K) Foxp3⁺ cell within CD4⁺ T cells (n = 5–6 mice per group, presented as mean ± SEM, ns p > 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001); (L and M) Analysis of innate-like T cells in the thymus.

(L) Frequencies of NK1.1⁺ T cells and (M) γδ T cells (n = 5 mice per group, presented as mean ± SEM. ns p > 0.05, ∗∗∗p < 0.001).

(N–P) Evaluation of peripheral T cells in situ.

(N) Absolute numbers of splenic T cells, (O) frequencies of CD44⁺CD62L⁻ cells and (P) IFNγ⁺ cells (n = 3 mice per group, presented as mean ± SEM. ∗p < 0.05, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001).

(Q and R) Cytokine production upon stimulation. Frequencies of (Q) IFNγ⁺ and (R) TNFα⁺ cells among peripheral naive T cells following ex vivo anti-CD3/CD28 stimulation (n = 3 mice per group, bar graphs show mean ± SEM. ∗p < 0.05, ∗∗p < 0.01). Data are representative of at least two independent experiments unpaired Students’t test was used to perform the statistical analysis. See also Figure S2.