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. 2026 Mar 11;29(4):115313. doi: 10.1016/j.isci.2026.115313

Figure 4.

Figure 4

Eif4g2 deletion specifically ablates the IL-7 response

(A and B) Single-cell transcriptomic landscape of thymocytes. UMAP visualization of (A) all thymic cells and (B) the subtypes of T cells, color-coded by cell type.

(C) Heatmap showing the expression pattern of 15 core signaling component genes from seven selected KEGG pathways across the cell clusters identified in (A and B).

(D–F) Functional response of CD4+CD8lo transitional cells to IL-7. Relative mRNA level of (D) Runx3 and (E) Bcl2 following 10 ng/ml IL-7 stimulation (n = 3, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001).

(F) Western blot analysis of key signaling pathway activation with or without IL-7 stimulation.

(G) Quantification of cell death in peripheral naive T cells under IL-7 stimulation ex vivo (n = 3, ∗∗∗∗p < 0.0001).

(H–J) TCR signaling evaluation in DP cells under stimulation with anti-TCRβ/CD2 ex vivo, including (H) representative plots of CD69 expression examination, (I) frequency of CD69+ cells and (J) cell death level (n = 4, ns p > 0.5). Data are representative of at least two independent experiments. Bar graphs show mean ± SEM and unpaired Students’t test was used to perform the statistical analysis. See also Figures S3–S5.