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Binding curves for the interaction of GTPγS with the light-activated rhodopsin-Gt complex in DOPC/DOPE (25:75 mol %) films. Isotherms plot the shifts in the resonance angle minimum of the PWR spectra obtained after addition of aliquots of GTPγS for p- (▪) and s-polarized (▴) light. Solid curves correspond to hyperbolic fits to the data; dissociation constant values are given in Table 1.
