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. 2004 Oct 8;88(1):255–268. doi: 10.1529/biophysj.104.043620

FIGURE 4.

FIGURE 4

1H NMR spectra (35°C) of magnetically aligned DMPC/DHPC (q = 4.5) + 1 mol % DMPE-PEG 2000 bicelles as a function of the field gradient pulse duration in the STE PFG NMR sequence. The gradient pulse amplitude was 244 G cm−1, whereas τ2 equaled 15 ms and τ1 equaled 400 ms. Gradient pulse durations, from front to back in units of ms: 0.1, 0.25, 0.50, 0.75, 1.0, 1.25, 1.5, 1.75, 2.0 2.5, 3.0, 3.5, 4.5, 5.5, 6.0, 6.5, and 7.0. The water resonance at 4.2 ppm decays rapidly due to water's fast diffusion. The combined DHPC choline methyl and DMPE-PEG 2000 headgroup resonance at 3.3 ppm decays far more slowly, as expected, for bilayer intercalated species.