FIGURE 8.
Electron micrographs of actin filaments after incubation with Myo1b. Myo1b and actin at a ratio of 1:10 (panel A) 1:15 (panel B) and 1:25 (panel C) were incubated in 10 mM Tris, pH 7.8, 100 mM KCl, 1 mM MgCl2, 1 mM EGTA, and 0.5 mm DTT overnight, applied to grids, and stained with uranyl acetate. A higher magnification of the area in panel A indicated by arrows is shown in panel A′ (bar, 0.2 μm). Control samples containing actin only are shown in panel D. Bundling of actin filaments was proportional to the Myo1b concentration. Bar (0.2 μm) in panel D applies to all panels except A′.