TABLE 2.
Molecules exhibiting strong correlations between spectral fit parameters
| Experimental conditions | |||||||
|---|---|---|---|---|---|---|---|
| Temperature (°C) | 20 | 20 | 20 | 20 | 20 | 28 | 35 |
| Excitation (μW) | 0.5 | 1.5 | 2.5 | 3.5 | 6 | 0.5 | 0.5 |
| Numbers of measured particles | |||||||
| Total | 149 | 124 | 128 | 119 | 138 | 120 | 94 |
| SDFP > 2.44 | 2 | 23 | 32 | 30 | 58 | 8 | 5 |
| Numbers of correlating particles | |||||||
| Amplitude-FLP positive | 1 | 11 | 7 | 9 | 19 | 1 | 0 |
| FLP-fwhm negative | 0 | 7 | 5 | 6 | 13 | 1 | 3 |
| Combination (blue shift) | 0 (0) | 6 (6) | 3 (2) | 5 (5) | 11 (9) | 0 (0) | 0 (0) |
| Amplitude-FLP negative | 0 | 3 | 3 | 13 | 7 | 2 | 1 |
| FLP-fwhm positive | 0 | 4 | 7 | 13 | 7 | 4 | 1 |
| Combination (red shift) | 0 (0) | 3 (3) | 2 (2) | 11 (11) | 5 (3) | 2 (2) | 0 (0) |
“Combination (blue shift)” refers to the number of particles that exhibit both amplitude-FLP and FLP-fwhm correlations with corresponding signs. Numbers in brackets point to the particles that, in addition to both pairwise correlations, have the negative third moment of the FLP time trace. Similarly, “combination (red shift)” relates to the corresponding combination of correlations and particles with positive third moment of the FLP time trace.