TABLE 1.
Dissociation constants and thermodynamic data for binding of drug to EmrE in various mimetic environments at 25°C
| Environment | Drug | KD (μM) | ΔG (kcal/mol) | ΔH (kcal/mol) | ΔS (kcal/mol per K) |
|---|---|---|---|---|---|
| SUV | Ethidium | 5.5 ± 2.1 | −7.2 ± 0.2 | −10.7 ± 0.1 | −11.8 ± 1.0 |
| Methyl viologen | 38.2 ± 8.7 | −6.1 ± 0.1 | −7.5 ± 0.7 | −4.9 ± 2.7 | |
| Proflavin | 10.7 ± 2.8 | −6.8 ± 0.2 | −8.9 ± 0.2 | −7.1 ± 1.0 | |
| TPP | Could not be determined | ||||
| SDS | Ethidium | 5.2 ± 1.4 | −7.2 ± 0.2 | −11.4 ± 0.1 | −14.0 ± 0.6 |
| Methyl viologen | 5.4 ± 1.2 | −7.2 ± 0.1 | −9.7 ± 0.2 | −8.4 ± 0.6 | |
| Proflavin | 4.5 ± 0.8 | −7.3 ± 0.1 | −10.6 ± 0.2 | −11.1 ± 0.9 | |
| TPP | 4.8 ± 0.8 | −7.3 ± 0.1 | −12.1 ± 0.1 | −16.0 ± 0.5 | |
| DM | Ethidium | 6.3 ± 1.0 | −7.1 ± 0.1 | −10.9 ± 0.1 | −12.7 ± 0.2 |
| Methyl viologen | 46.2 ± 10.5 | −5.9 ± 0.1 | −7.7 ± 1.6 | −7.1 ± 4.7 | |
| Proflavin | 5.2 ± 0.9 | −7.2 ± 0.1 | −9.6 ± 0.1 | −7.8 ± 0.5 | |
| TPP | 25.5 ± 6.2 | −6.3 ± 0.1 | −9.9 ± 0.5 | −12.2 ± 2.2 | |
Each value represents three separate trials.