Table 3.

Intra-day and inter-day accuracy and precision of 2-dC, 5-mdC, and 5-hmdC in negative control.

Compound Name	QC	Nominal Concentration (ng/mL)	Intra-day (n=6)			Inter-day (n=18)
			Observed Concentration (Mean ± SD)	Accuracy (RE %)	Precision (CV %)	Observed Concentration (Mean ± SD)	Accuracy (RE %)	Precision (CV %)
2-dC	LLOQ	5	5.0 ± 0.2	0.9	4.62	4.9 ± 0.23	−2.68	4.76
	LQC	15	14.6 ± 0.2	−2.44	1.27	14.5 ± 0.44	−3.52	3.02
	MQC	500	486.5 ± 10.8	−2.7	2.21	500.2 ± 18.64	0.04	3.70
	HQC	4000	3775 ± 214.6	−5.63	5.69	3917 ± 167.2	−2.08	4.27
5-mdC	LLOQ	0.5	0.5 ± 0.03	0.67	6.50	0.47 ± 0.03	−5.73	6.77
	LQC	1.5	1.60 ± 0.02	6.67	1.19	1.50 ± 0.07	−0.22	4.47
	MQC	50	47.40 ± 1.5	−5.20	3.14	50.37 ± 1.99	0.76	3.93
	HQC	400	382.8 ± 33.01	−4.29	8.62	391.8 ± 22.47	−2.04	5.74
5-hmdC	LLOQ	0.05	0.05 ± 0.004	−5.03	9.25	0.05 ± 0.003	−8.81	7.77
	LQC	0.15	0.15 ± 0.009	3.00	5.69	0.14 ± 0.01	−3.98	6.83
	MQC	1	0.94 ± 0.058	−6.05	6.15	0.98 ± 0.06	−1.46	5.63
	HQC	8	7.59 ± 0.448	−5.13	5.91	7.40 ± 0.35	−7.50	4.81