FIGURE 5.

Root mean-square deviation of Nanodiscs. MSP1 shown in green, MSP1 Δ(1–11) in black, MSP1 Δ(1–22) in red, and MSP1 Δ(1–22)g in blue. (a) RMSD of Nanodiscs relative to the initial structure. (Inset) Mean-square deviation of the DPPC lipid bilayer bounded by the membrane scaffold proteins over time. For the lipid bilayer RMSD to reach its asymptotic value, i.e., equilibrium, requires ∼10 μs (diffusion coefficient 1.5 nm²/μs). (b) RMSD measuring the out-of-plane deformation of the membrane scaffold proteins, determined through the deviation of all atoms in each membrane scaffold protein relative to the plane bisecting the Nanodisc.