TABLE 1.

Membrane scaffold proteins used for simulation of Nanodiscs

Scaffold protein name	Protein deletions	Alignment of scaffold proteins	Simulation length (ns)
MSP1*	-	K90/K90	4.2
MSP1 Δ(1–11)	1–11	K90/K90	4.5
MSP1 Δ(1–22)	1–22†	K90/K90	6.9
MSP1 Δ(1–22)g	1–22†	G23/Q200	4.5

^*The MSP1 membrane scaffold protein contains 200 amino acid residues. The sequence can be found in the supporting information of Denisov et al. (2004).

^†MSP1 Δ(1–22) and MSP1 Δ(1–22)g contain a proline to serine mutation at residue 23.