Table 1.
Anchorage-independent growth and tumor formation
| Cell type | Soft agar
|
Tumors | ||
|---|---|---|---|---|
| 103 | 104 | 105 | ||
| Vector-GFP | 4 | 10 | 39 | 0/9 |
| TERT-GFP | 11 | 20 | 20 | 0/9 |
| Vector-Ras | 10 | 91 | 1,564 | 1*/12 |
| TERT-Ras | 5 | 62 | 1,558 | 12/12 |
1 × 103, 1 × 104, and 1 × 105 cells were plated in soft agar and colony formation was assessed 4 weeks later. Sublethally irradiated mice were injected s.c. with 2 × 106 cells in three independent sites and tumor formation was monitored weekly. Numbers in the tumor column represent the number of tumors/number of injection sites that formed after 10 weeks.
*
Tumor formed after an extended latency and remained less than 3 mm in diameter.