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. 2026 Mar 23;17:1707493. doi: 10.3389/fimmu.2026.1707493

Figure 4.

Scientific figure contains multiple panels: A shows a Venn diagram comparing metabolite overlaps between CLP, CLP+Blautia, and Sham groups; B displays a PLS-DA plot clustering metabolic profiles; C is a bar graph of the top twenty KEGG pathways by metabolite count; D presents two volcano plots of significantly altered metabolites between groups; E is a KEGG enrichment bubble plot, highlighting impacted pathways with dots scaled by gene ratio and color-coded by p-value; F shows a heatmap of metabolite abundance across the three groups for various tryptophan metabolites; G summarizes VIP values and heatmaps for specific metabolites, focusing on indole-3-acetic acid; H is a box plot comparing indole-3-acetic acid levels, indicating statistically significant group differences.

Blautia-derived IAA is involved in the pathophysiological process of SI-ALI development. (A) Venn diagram of metabolites shared and unique among Sham, CLP, and Blautia-Pretreated CLP rats. (B) PLS-DA scatter plot of fecal metabolomes: Sham, CLP, and Blautia-Pretreated CLP rats. (C) Top 20 differentially enriched metabolic pathways in gut microbiota gene sets among cohorts. (D) Differential fecal metabolite analysis: Sham vs. CLP-Septic Rats and CLP-vs. Blautia-Intervention Cohorts (Volcano plot representation). (E) KEGG enrichment analysis of intestinal metabolites: CLP group versus Blautia-Pretreated CLP group. (F) Heatmap visualization of differential metabolites: Sham-operated, CLP group, and Blautia-Pretreated CLP groups (color key: blue = downregulation; red = upregulation). (G) Discriminant metabolites identified by VIP scoring. (H) Quantification metabolomics of IAA levels. ** P<0.01.