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. 2004 Nov 19;88(2):1018–1029. doi: 10.1529/biophysj.104.052043

FIGURE 4.

FIGURE 4

Effect of external [K+] on TEA affinity. (A) Amplitude histograms generated at −75 mV with intracellular 20K4 and 20, 200, or 1000 mM extracellular K+ at pH 7.0, under control conditions (top) or in the presence of 10 mM TEA (bottom). Histograms were fitted with the sum of two Gaussians (one each to represent the closed and open states, solid lines). The decreased amplitude caused by TEA is reflected by the shift in the maximum amplitude of the open state—the control maximum is designated by the dashed line; the position of the maximum in TEA is shown with the solid line. (B) K1/2-voltage relation plotted for the three different extracellular [K+] (1000 mM, triangles; 200 mM, squares; 20 mM, circles). Data show mean ±SE of 3–4 independent determinations.