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Expression of mammalian InsP3R isoforms in Sf9 cells. Western blot of microsomal proteins. Microsomes isolated from noninfected Sf9 cells (Sf9) and from Sf9 cells infected with RT1, RT2, and RT3 baculoviruses were analyzed by Western blotting with polyclonal antibodies specific for InsP3R1 (A), InsP3R2 (B), and InsP3R3 (C) as indicated. For each microsomal preparation, 10 μg of total protein was loaded on the gel.
