Electrophoretic mobility of the permuted sequence isomers of SV40 in: (A) 1% agarose and (B) 5.7% T, 1% C polyacrylamide gels. The leftmost lanes in panel A contain the 1-kb ladder and the HinfI digest of pBR322 as mobility markers; the rightmost lane in panel B contains the 1-kb ladder. The other lanes contain SV40 linearized with BanI, HpaII, PpuMI, EcoRV, HaeII, DrdI, AccI, AflII, EcoRI, XcmI, Bsp120I, BamHI, and BclI, from left to right.