FIGURE 6.

Semilogarithmic plot of DNA molecular weight, N, in basepairs, as a function of the electrophoretic mobility, μ, observed in a 6.9% T, 3% C polyacrylamide gel. (○) Normal fragments in the 50-bp ladder; (□) SV40 fragments without the curvature module or with the apparent bend center located very close to one end; (▵) insertion derivatives that are significantly curved in free solution; and (▴) insertion derivatives that are not curved, or only slightly curved, in free solution (see text). The drawn line corresponds to the fitting function describing the migration of the fragments in the 50-bp ladder. Selected fragments are identified for ease of reference.