TABLE 4.

Characterization of insertion derivatives

Fragment Name	Insertion site	Inserted sequence	Insertion size, bp	μ-Decrement	Average τ-decrement*
204B†	1926	AGCTA	5	−3	−4 ± 5
204C†	1942	AGCTA	5	−3	−4 ± 3
205	1988	CCATCGATGG	10	−24	−17 ± 2
212	1913	TGCCATCGATGG	13	−13	−5 ± 1
213	1950	TTCCATCGATGGAA	14	−12	−6 ± 2
217	1988	Nested ClaI linker dimers‡	22	−24	−8 ± 1
224	1913	Nested ClaI linker dimers‡	25	−13	−2 ± 2
225	1950	Nested ClaI linker dimers‡	26	−14	+1 ± 3
227	1913, 1950	Two insertions§	14	−7	+1 ± 2
231	1913	Tandem ClaI linker trimers¶	32	−17	−6 ± 1
236	1913	Nested ClaI linker trimers‖	37	−13	−2 ± 2

^*Average τ-decrement observed in buffers B, C, and E and standard deviation.

^†Also, A₆ and T₇ replaced by TCTAGA.

^‡Inserted sequence, CCATCGCCATCGATGGCGATGG.

^§Inserted sequences, CCATCGATGGCATG at 1913 and TTCCATCGATGGAA at 1950.

^¶Inserted sequence, GCCCATCGATGGCCATCGATGGCCATCGATGG.

^‖Inserted sequence, ATGCCATCGCCATCGCCATCGATGGCGATGGCGATGG.