Skip to main content
. 2004 Dec 1;88(2):1207–1214. doi: 10.1529/biophysj.104.051771

FIGURE 5.

FIGURE 5

Excision of the 1,2-GG intrastrand cross-link of cisplatin and the monofunctional adducts of [PtCl(dien)]Cl or [PtCl(NH3)3]Cl by rodent excinuclease. The substrates were incubated with CHO AA8 CFE for 40 min at 30°C and subsequently treated overnight with NaCN before analysis in 10% polyacrylamide/8 M urea denaturing gel. Lanes are: 1 (cisPt), the substrate containing the 1,2-GG intrastrand cross-link of cisplatin formed in the TGGT sequence; 2 (TGT-dienPt), the substrate containing the monofunctional adduct of [PtCl(dien)]Cl formed in the TGT duplex; 3 (AGT-dienPt), the substrate containing the monofunctional adduct of [PtCl(dien)]Cl formed in the AGT duplex; 4 (no Pt), control, unplatinated substrate.