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. 2004 Dec 1;88(2):1207–1214. doi: 10.1529/biophysj.104.051771

TABLE 1.

Calorimetrically derived thermodynamic parameters for dissociation (melting) of the 15-bp duplexes unmodified or containing a single, site-specific monofunctional adduct of [PtCl(dien)]Cl or [PtCl(NH3)3]Cl

Duplex Tm (°C) ΔHcal (kcal/mol) ΔS (cal/mol) Inline graphic ΔHvH (kcal/mol) ΔHvHHcal Inline graphic
TGT 57.5 97.1 295.5 9.0 102.6 1.06 7.42
TGT-Pt(dien) 49.5 79.9 248.6 5.8 93.8 1.17 5.86
TGT-Pt(NH3)3 49.4 81.9 254.2 6.1 93.0 1.14 5.94
AGT 57.5 94.9 288.8 8.8 105.1 1.11 7.14
AGT-Pt(dien) 55.6 88.7 271.1 7.9 101.7 1.15 5.74
AGT-Pt(NH3)3 53.6 85.0 262.6 6.7 91.2 1.07 5.46

The ΔH, ΔS, and Inline graphic values are averages derived from three independent experiments. The experimental uncertainties of the parameters are as follows: Tm (±0.5°C), ΔHcal (±2%), ΔS (±3%), Inline graphic