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Individual transient decays for two-photon excitation at 890 nm and emission at 500 nm (±20 nm), indicating contamination of the measurement of CXCR4-EGFP in the presence of full-length PKCα-DsRed in unstimulated cells. (A) PKCα-DsRed, bi-exponential kinetics are indicated. (B) CXCR4-EGFP alone; single-exponential kinetics are observed. (C) PKCα-DsRed and CXCR4-EGFP, Kinetics observed are contaminated with the fast component of PKCα-DsRed.
